Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 857, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2019.113772
Keywords
Branched chain amino acids; Clinical analysis; Capillary electrophoresis; Coating; Contactless conductivity detection
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Funding
- Czech Science Foundation [17-12648S]
- Czech Ministry of Health [19-01-00101]
- Czech Academy of Sciences [RVO 61388963]
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Plasmatic levels of valine, isoleucine, leucine, alanine and glutamine were determined by capillary electrophoresis with contactless conductivity detection. A new kind of covalently coated capillary based on polyacrylamide and (3-acrylamidopropyl) trimethylammonium chloride was employed. The amino acids were separated in the counter-current regime, in which the analytes migrate under counter-current electro-osmotic flow with a value of 5.53 . 10(-9) m(2) V-1 s(-1) in the background electrolyte, 3.2 mol/L AcOH +10% v/v MeOH. The baseline separation of all five amino acids from other plasmatic compounds was achieved in <5 min: the limits of detections are 0.13-0.14 mu mol/L for the treated sample and 0.50-0.58 mu mol/L for the untreated plasma. The method was used to measure venous-arterial differences of amino acids in hyperinsulinemic patients with cachexia. (C) 2019 Elsevier B.V. All rights reserved.
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