Journal
JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 122, Issue 9, Pages 1003-1008Publisher
WILEY
DOI: 10.1002/jcb.29637
Keywords
chondrocytes; LUADT1; miR-34a; osteoarthritis; SIRT1
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Funding
- China Postdoctoral Science Foundation [2019M651898]
- Changzhou Science and Technology Program [CJ20180057]
- Project of Changzhou Medical Innovation team [CCX201807]
- High-level Medical Talents Training Project of Changzhou [2016CZLJ007]
- Natural Science Foundation of the Jiangsu for Youth [BK20180182]
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The study discovered that LUADT1 and miR-34a play a crucial role in osteoarthritis by directly interacting with each other and participating in the regulation of chondrocyte apoptosis through SIRT1.
It is known that miR-34a can promote the apoptosis of chondrocytes, which directly contribute to osteoarthritis (OA). Through bioinformatics analysis, we found that long noncoding RNA LUADT1 may interact with miR-34a. We, therefore, further investigate the interactions between them in osteoarthritis. We found that LUADT1 was downregulated, while miR-34a was upregulated in OA synovial fluid. Correlation analysis revealed no significant correlation between them. Overexpression experiment also revealed no significant effects of LUADT1 and miR-34a on the expression of each other. However, the dual-luciferase assay showed that LUADT1 and miR-34a can directly interact with each other. Moreover, LUADT1 overexpression led to the upregulation of SIRT1, which is a downstream target of miR-34a. Cell apoptosis showed that LUADT1 and SIRT1 overexpression led to decreased, while miR-34a led to increased apoptotic rates of chondrocytes. Therefore, LUADT1 regulates miR-34a/SIRT1 to participate in chondrocyte apoptosis.
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