Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 24, Issue 2, Pages 1878-1892Publisher
WILEY
DOI: 10.1111/jcmm.14883
Keywords
drug resistance; Kruppel-like factor 4; LINC00673; methylation; proliferation; prostate cancer
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Funding
- Prostate Cancer Foundation China (PCF China) Young Investigator Award
- National Natural Science Foundation Youth Fund [81001143]
- Shenyang Science and Technology Program Key Science and Technology Research and Development Program [17-230-9-18]
- National Key Research and Development Program of China [2017YFC0908003]
- First Batch of Talents of Introduction of Top Health Talented Team of Qinghai Province
- First Batch of Talents of Xining Absorbing 555 Talents Project
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Prostate cancer is one of the major causes of cancer-related mortality in men across the world. Recently, long non-coding RNAs (lncRNAs) and Kruppel-like factor 4 (KLF4) have been reported to participate in the biology of multiple cancers including prostate cancer. Here, this study aimed to explore the possible role of LINC00673 in prostate cancer via KLF4 gene promoter methylation. Microarray-based gene expression profiling of prostate cancer was employed to identify differentially expressed lncRNAs and genes, after which the expression of LINC00673 and KLF4 in prostate cancer tissues was determined using RT-qPCR. Next, the relationship between LINC00673 and KLF4 was evaluated using in silico analysis. Further, the effect of LINC00673 and KLF4 on cell proliferation and drug resistance of transfected cells was examined with gain- and loss-of-function experimentation. It was found that LINC00673 was highly expressed, while KLF4 was poorly expressed in prostate cancer tissues. Additionally, LINC00673 could bind to KLF4 gene promoter region and recruit methyltransferase to the KLF4 gene promoter region. Moreover, LINC00673 silencing was demonstrated to reduce methylation of the KLF4 gene promoter to elevate the expression of KLF4, thus suppressing the proliferation and drug resistance of prostate cancer cells. In summary, LINC00673 silencing could drive demethylation of the KLF4 gene promoter and thus inhibit the proliferation and drug resistance of prostate cancer cells, suggesting that silencing of LINC00673 and elevation of KLF4 could serve as tumour suppressors in prostate cancer.
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