Journal
JOURNAL OF BIOPHOTONICS
Volume 13, Issue 3, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201960085
Keywords
adipose; fetus; metabolism; OXPHOS; redox; skeletal muscle; two photon microscopy
Categories
Funding
- Australian Research Council [FT170100431]
- National Health and Medical Research Council [APP1066916]
- Australian Government Research Training Program
- Australian Research Council [FT170100431] Funding Source: Australian Research Council
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The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two-photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism.
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