Efficacy of miltefosine therapy against subcutaneous experimental pythiosis in rabbits

We evaluated the in vitro activity of miltefosine against 29 Pythium spp. and the in vivo therapeutic response of 2 mg/kg/day of miltefosine given orally to rabbit with pythiosis induced experimentally. The MICs (in mg/mL) of miltefosine was medium-dependent and ranged from 0.5 to 2 and 32-64 on RPMI 1640 and Mueller Hinton broth, respectively. The treatment with miltefosine demonstrated significantly lower subcutaneous lesion areas compared to the control group but was not sufficient for the complete remission of the lesions. This study indicates that miltefosine has limited efficacy against pythiosis and furthers in vitro and in vivo studies are necessary to determine the possible potential of this drug in the treatment of pythiosis. (C) 2019 Elsevier Masson SAS. All rights reserved.