Glycomyces buryatensis sp. nov., an actinobacterium isolated from steppe soil

A novel actinobacterial strain, designated 18(T), was isolated from a steppe soil sample collected in Buryatia, Russia and subjected to polyphasic taxonomic characterization. The strain was aerobic and Gram-stain-positive. The 16S rRNA gene sequence of strain 18(T) exhibited highest similarity to Glycomyces paridis CPCC 20435(T) (97.2 %). Results of phylogenetic analysis showed that strain 18(T) formed a distinct branch clearly affiliated to the genus Glycomyces. Whole-cell hydrolysates of the isolate contained meso-diaminopimelic acid as the cell wall diamino acid. The whole-cell sugar profile was found to contain galactose, glucose, ribose and xylose. MK-10(H-4) and MK-11(H-4) were the predominant menaquinones. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and four unidentified polar lipids. The major fatty acids identified were anteiso-C-15:0 and iso-C15:0(, )The G+C content of the genomic DNA of strain 18(T) was 68.0 mol% (draft genome sequence). Based on its phylogenetic, phenotypic and chemotaxonomic features, strain 18(T) was considered to represent a novel species of the genus Glycomyces, for which the name Glycomyces buryatensis sp. nov. is proposed. The type strain of Glycomyces buryatensis is 18(T) (=JCM 33362(T) =CGMCC 4.7610(T)).