Vitreimonas flagellata gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from an activated sludge sample

A coccobacilli-shaped proteobacterium, designated strain SYSU XM001(T), was isolated from an activated sludge sample collected from JiMei sewage treatment plant, Xiamen, PR China. Cells were Gram-stain-negative, catalase-positive and oxidasenegative. The 16S rRNA gene sequence of strain SYSU XM001(T) shared less than 92% sequence identities with members of the class Alphaproteobacteria, with highest sequence similarity to Aquidulcibacter paucihalophilus TH1-2(T) (91.6%; family Hyphomonadaceae, order Rhodobacterales). The strain exhibited growth at 25-37 degrees C, pH 7.0-9.0 and in the presence of up to 1% (w/v) NaCl. Its chemotaxonomic features included ubiquinone-10 as the respiratory isoprenologue, iso-C-16(:0), 10-methyl C-16:0 TSBA and anteiso-C-17:0 as major cellular fatty acids and monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids as the main polar lipids. The DNA G+C content was determined to be 62.9% (draft genome). Analyses of the phylogenetic data and differences in the chemotaxonomic and biochemical features from related genera in the family Hyphomonadaceae indicated that strain SYSU XM001(T) merits representation of a novel species of a novel genus, for which the name Vitreimonas flagellata gen. nov., sp. nov. is proposed. The type strain of Vitreimonas flagellata is SYSU XM001(T) (=CGMCC 1.16661(T)=KCTC 62915(T)).