Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/ijms21051626
Keywords
Rubisco; Rubisco activase; Rubisco activation; photosynthesis improvement; nitrogen; transgenic rice
Funding
- Japan Society for the Promotion of Science: [JSPS] [19J10653, 18H02111, 16H06379]
- Core Research for Environmental Science and Technology (CREST) Scientific Research Grant from the Japan Society for Technology (JST) [JPMJCR15O3]
- Grants-in-Aid for Scientific Research [18H02111, 16H06379, 19J10653] Funding Source: KAKEN
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It has been reported that overproduction of Rubisco activase (RCA) in rice (Oryza sativa L.) decreased Rubisco content, resulting in declining photosynthesis. We examined the effects of RCA levels on Rubisco content using transgenic rice with overexpressed or suppressed RCA under the control of different promoters of the RCA and Rubisco small subunit (RBCS) genes. All plants were grown hydroponically with different N concentrations (0.5, 2.0 and 8.0 mM-N). In RCA overproduced plants with > 2-fold RCA content (RCA-HI lines), a 10%-20% decrease in Rubisco content was observed at 0.5 and 2.0 mM-N. In contrast, at 8.0 mM-N, Rubisco content did not change in RCA-HI lines. Conversely, in plants with 50%-60% increased RCA content (RCA-MI lines), Rubisco levels remained unchanged, regardless of N concentration. Such effects on Rubisco content were independent of the promoter that was used. In plants with RCA suppression to < 10% of the wild-type RCA content, Rubisco levels were increased at 0.5 mM-N, but were unchanged at 2.0 and 8.0 mM-N. Thus, the effects of the changes in RCA levels on Rubisco content depended on N supply. Moreover, RCA overproduction was feasible without a decrease in Rubisco content, depending on the degree of RCA production.
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