Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/ijms21041481
Keywords
lettuce; germination; thermoinhibition; thermal stress; metabolomics
Funding
- Shanghai Science and Technology Support Project [19391900600]
- Seed Industry Development Project of Shanghai, China [2016, 1-8]
- Sharing Platform for National Crop Germplasm Resources (Shanghai) [NICGR2019-21]
- Shared Platform of Crop Germplasm Resources in Shanghai [18DZ2293700]
- Central Public-Interest Scientific Institution Basal Research Fund [Y2020XK01, Y2018YJ25]
- National Agriculture Science and Technology Center, Chengdu [NASC2019AR01]
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Temperature strongly influences lettuce (Lactuca sativa L.) seed germination. Different lettuce genotypes respond differently to higher temperatures or thermal stress. In this study, we evaluated the germination performance of 304 lettuce accessions incubated at three temperature settings, 21 degrees C, 28 degrees C and 35 degrees C, respectively, for 40 h. At 21 degrees C, seeds of all 304 accessions germinated with very well an average germination percentage of 87.72%; at 28 degrees C, the average germination percentage dropped to 42.84% and at 35 degrees C, the germination decreased to 1.01%. Then, we investigated changes in metabolic profiles of lettuce seed response to thermal stress using an untargeted metabolomics approach. Results suggested that seeds of thermal-sensitive and thermal-tolerant cultivars employed different metabolic strategies in response to thermal stress during germination. Thermal-sensitive buds accumulated more significant amounts of organic acids, amino acids, sugars, sterols, phenolic compounds and terpenoids compared to thermal-tolerant buds at 21 degrees C. Thermal-tolerant lettuce cultivar accumulated higher concentrations of amino acids, organic acids, sugars, sesquiterpene lactones, sterols, and fatty acids derivatives during the germination at 35 degrees C compared to germinated at 21 degrees C. This investigation paves the way to link the metabolomics to other external and internal factors affecting lettuce seed germination under thermal stress.
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