Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/ijms21010316
Keywords
Dendrobium; molecular identification; endophytic fungi; pathogenicity; protocorm; seedling
Funding
- Distinguished High-Level Talents Research Grant from a Guizhou Science and Technology Corporation Platform Talents Fund [[2017]5733-001, CK-1130-002]
- National Natural Science Foundation of China [31560079]
- Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture [XTD1825]
- Cultivation of Academic New Seedlings and Exploration of Innovative Specialties [CK-1130-012]
- Scientific and Technological Projects in Honghuagang District of Zunyi City [(2018):10]
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Dendrobium are tropical orchid plants that host diverse endophytic fungi. The role of these fungi is not currently well understood in Dendrobium plants. We morphologically and molecularly identified these fungal endophytes, and created an efficient system for evaluating the pathogenicity and symptoms of endophytic fungi on Dendrobium nobile and Dendrobium officinale though in vitro co-culturing. ReThe colony morphological traits of Dendrobium myco-endophytes (DMEs) were recorded for their identification. Molecular identification revealed the presence of Colletotrichum tropicicola, Fusarium keratoplasticum, Fusarium oxysporum, Fusarium solani, and Trichoderma longibrachiatum. The pathogenicity results revealed that T. longibrachiatum produced the least pathogenic effects against D. nobile protocorms. In seedlings, T. longibrachiatum showed the least pathogenic effects against D. officinale seedlings after seven days. C. tropicicola produced highly pathogenic effects against both Dendrobium seedlings. The results of histological examination of infected tissues revealed that F. keratoplasticum and T. longibrachiatum fulfill Koch's postulates for the existence of endophytes inside the living tissues. The DMEs are cross-transmitted inside the host plant cells, playing an important role in plant host development, resistance, and alkaloids stimulation.
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