Phenolic compounds and major steviol glucosides by HPLC-DAD-RP and invitro evaluation of the biological activity of aqueous and ethanolic extracts of leaves and stems: S. rebaudiana Bertoni (creole variety INIFAP C01) S. rebaudiana Bertoni (creole variety INIFAP C01): Bioactive compounds and Functionality

The number and use of medicines based on natural products such as plants have increased as scientific evidence indicates that they have various bioactive compounds with biological activities. This study determined the content of five phenolic compounds and two major steviol glucosides in S. rebaudiana (INIFAP-C01 variety): Extracts from Muna: aqueous and ethanolic extracts of leaves and stems (HAMU and HEMU) (TAMU and TEMU); and extracts from Mococha: aqueous and ethanolic extracts of leaves and stems (HAMO and HEMO) (TAMO and TEMO). The HEMU extract had a higher content of phenolic compounds (2.23 g/100g) and major steviol glucosides (17.12 g/100g). HEMU and HEMO extracts showed a higher inhibition of Angiotensin-Converting Enzyme (ACE) activity (95.04% and 91.73%, respectively), alpha-amylase activity (52.85% and 86.78%, respectively), and alpha-glucosidase activity (94.53% and 91.73%, respectively). Furthermore, HEMU and HEMO extracts showed higher antioxidant capacities (79.95% and 76.64%, respectively), and the HEMU extract achieved a Trolox Equivalent Antioxidant Capacity (TEAC) value of 174 mol/g. Creole variety (INIFAP C01) S. rebaudiana has phytochemicals able to help promote health, inhibit enzymes in metabolism, and provide antioxidant protection.