Journal
INTERNATIONAL JOURNAL OF CARDIOLOGY
Volume 296, Issue -, Pages 136-140Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.ijcard.2019.08.024
Keywords
Ischemia/reperfusion injury; Extracellular vesicles; Circular RNA; MicroRNA
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Funding
- National Natural Science Foundation of China (NSFC) [81670458, 81370434, 81470393]
- Shanghai Municipal Health and Family Planning Commission [(2018-2020)-FWTX-2007]
- Three-year plan of Pudong Health Bureau of Shanghai [PWYgf2018-05]
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Background: Increasing studies indicated the involvement of extracellular vesicles (EVs) in cardiovascular diseases. However, the role of circular RNAs (circRNAs) in cardiac EVs (cEVs) during ischemia/reperfusion (I/R) injury remain unclear. Methods: We isolated the cEVs from I/R injured hearts and performed RNA sequencing (RNA-seq) to identify the profile of circRNA in cEVs and investigated their potential roles in I/R pathological process. Results: Cardiac I/R induced a significantly elevated release of EVs in heart within 24 h. RNA-seq of cEVs identified 185 significantly differentially expressed (DE) circRNAs including 119 down-regulated and 66 up-regulated circRNAs in I/R group compared with the sham. GO and pathway analysis showed that these DE-circRNAs were associated with protein binding and kinase activator activity and mainly involved in the metabolic process. The circRNA-miRNA analysis exhibited the broad potentials of the DE-circRNAs to regulate target genes by acting on the miRNAs. Conclusions: These findings revealed for the first time the specific expression pattern of circRNAs in EVs derived from sham and I/R heart tissues and provided some potential targets and pathways involving in I/R injury which may provide important evidences for the role of both circRNA and EVs in the pathology of cardiac I/R. (C) 2019 Elsevier B.V. All rights reserved.
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