Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 143, Issue -, Pages 341-348Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.12.031
Keywords
Chitosan; Sporothrix brasiliensis; Biofilm
Funding
- Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico - FUNCAP, Brasil [PNE-0112-00049.01.00/16]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/Programa de Cooperacao Academica em Defesa Nacional (CAPES), Brasil [27505/2016]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasil [305024/2017-5]
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Sporotrichosis, caused by Sporothrix schenckii complex species, is the most prevalent subcutaneous mycosis in many areas of Latin America. Chitosan has been used as an antifungal agent; however the effects of the molecular weight (MW) of chitosan (i.e. high (HMW), medium (MMW) and low (LMW) molecular weight chitosan) on S. brasiliensis has not been well described, particularly on biofilms. Effects on the planktonic form activity of S. brasiliensis were quantified by broth microdilution, while anti-biofilm activity was quantified by measuring metabolic activity via XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide and biomass formation (crystal violet). The molecular weight of chitosan modulated its effect on the planktonic form of S. brasiliensis, presenting lower MIC values for LMW chitosan. With regards both the adhesive and mature phases of biofilm, the LMW chitosan reduced biomass and metabolic activity most effectively. This study confirms the effects of the molecular weight and deacetylation degree of chitosan on its antifungal properties for potentially pathogenic fungi. (C) 2019 Elsevier B.V. All rights reserved.
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