4.7 Article

Novel transglycosylation activity of β-N-acetylglucosaminidase of Lecanicillium lecanii produced by submerged culture

Journal

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 145, Issue -, Pages 759-767

Publisher

ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.12.237

Keywords

Transglycosylation; N-acetylglucosaminidase; Lecanicillium lecanii

Funding

  1. Secretary of Education, Science Technology and Innovation of Mexico City (SECTEI) of Government of Mexico City [SECTEI/196/2019]
  2. Consejo Nacional de Ciencia y Tecnologia (CONACyT)

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N-acetylglucosaminidase produced from Lecanicillium lecanii on submerged culture displayed hydrolytic and transglycosylation activities. The highest specific activity for the enzyme was 1.87 U/mg after 120 h of culture. The chromatographic purification for a single protein fraction showed a molecular weight of 50.4 kDa and hydrolytic N-acetylglucosaminidase activity of 17.59 U/mg at 37 degrees C and pH 6. This enzyme was able to transglycosylate and to synthesize oligosaccharides from 2 to 6 units with a degree of acetylation between 100 and 26% employing glucose, mannose, N-acetyl-D-glucosamine and N-acetyl-D-lactosamine as donor substrates. Optimal conditions of temperature and pH were determined for both types of enzymatic activities.

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