Fabrication of bioinspired chitosan/gelatin/allantoin biocomposite film for wound dressing application

A biocomposite film composed of biopolymers chitosan (CS), gelatin (GE) and allantoin (AT) was fabricated by solution casting technique. The functional group interaction of the biocomposite films was inspected through the Attenuated total reflectance Fourier transform spectrometer (ATR-FTIR). The morphological changes and crystallinity of biocomposite films with varied ratios of chitosan/gelatin (CS/GE) and allantoin were examined under Scanning electron microscope (SEM) and X-ray diffractometer (XRD). The water-absorbing capacity was found enhanced by an increase in the chitosan ratio. The biocomposite films exhibit good antioxidant, anti-inflammatory properties. The biocomposite films also display enhanced stability with steady degradation under the PBS medium. The biocomposite films reveal improved antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). Blood compatibility studies explore the non-hemolytic nature. The in vitro cytotoxicity by MIT assay shows excellent biocompatibility. The fibroblast adhesion on the biocomposite film displays enhanced proliferation and viability. These significant biological properties of biocomposite film make it an appropriate candidate for wound dressing application. (C) 2020 Elsevier B.V. All rights reserved.