Journal
INFLAMMATORY BOWEL DISEASES
Volume 26, Issue 7, Pages 974-984Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/ibd/izz328
Keywords
eosinophils; intestinal permeability; irritable bowel syndrome; mast cells; ulcerative colitis
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Funding
- Bengt Ihre research Scholarship
- AFA research foundation
- Bengt-Ihre fonden, County Council of Ostergotland
- Swedish Research Council (VR-Medicine and Health) [2017-02475]
- Swedish Foundation For Strategic Research [RB13-016]
- Instituto de Salud Carlos III
- Fondo de Investigacion Sanitaria [PI16/00583]
- Ministerio de Ciencia, Innovacion y Universidades, Spain [CIBER-EHD CB06/04/0021]
- Swedish Research Council [2017-02475] Funding Source: Swedish Research Council
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Background: Barrier dysfunction is recognized as a pathogenic factor in ulcerative colitis (UC) and irritable bowel syndrome (IBS), but it is unclear to what extent the factors related to barrier dysfunction are disease-specific. The aim of this study was to compare these aspects in UC patients in remission, IBS patients, and healthy controls (HCs). Methods: Colonic biopsies were collected from 13 patients with UC in remission, 15 patients with IBS-mixed, and 15 HCs. Ulcerative colitis patients had recently been treated for relapse, and biopsies were taken from earlier inflamed areas. Biopsies were mounted in Ussing chambers for measurements of intestinal paracellular permeability to (51)chromium (Cr)-ethylenediaminetetraacetic acid (EDTA). In addition, biopsies were analyzed for mast cells and cosinophils by histological procedures, and plasma tumor necrosis factor (TNF)-alpha was assessed by ELISA. Results: Ussing chamber experiments revealed an increased( 51)Cr-EDTA permeability in UC and IBS (P < 0.05). The Cr-51-EDTA permeability was higher in UC compared with IBS (P < 0.005). There were increased numbers of mucosal mast cells and eosinophils in IJC and IBS and more eosinophils in UC compared with IBS (P < 0.05). Also, increased extracellular granule content was found in UC compared with HCs (P < 0.05). The Cr-51-EDTA permeability correlated significantly with eosiuophils in all groups. Plasma TNF-alpha concentration was higher in UC compared with IBS and HCs (P < 0.0005). Conclusions: Results indicate a more permeable intestinal epithelium in inactive UC and IBS compared with HCs. Ulcerative colitis patients, even during remission, demonstrate a leakier barrier compared with IBS. Both eosinophil numbers and activation state might be involved in the increased barrier function seen in UC patients in remission.
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