Journal
BIORESOURCE TECHNOLOGY
Volume 194, Issue -, Pages 247-255Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.07.019
Keywords
Polyhydroxyalkanoates; Corn stover; Fungal cellulase cocktail; PHA synthase gene (phaC); Paracoccus sp LL1
Funding
- Basic Science Research Program of the National Research Foundation of Korea [NRF-2013R1A2A2A01067117]
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Bioprocessing of lignocellulose as a renewable resource for fuels, chemicals or value added products is a necessity to fulfil demands of petroleum products. This study aims to convert corn stover to polyhydroxyalkanoates (PHA). Corn stover was hydrolyzed to crude sugars by an on-site prepared cellulase cocktail from co-culture of Trichoderma reesei and Aspergillus niger. The potent PHA producer, Paracoccus sp. LL1, was isolated from Lonar Lake, India and could accumulate PHA up to 72.4% of its dry cell weight. PHA production reached 9.71 g/L from corn stover hydrolysate containing 40 g/L sugar mixture. The PHA synthase gene (phaC) sequence of the isolate showed 79% identity with the phaC gene of Paracoccus seriniphilus (E71) strain from the NCBI database. The nature/type of PHA was found to be poly(3-hydroxybutyrate) by Fourier transform infrared spectroscopy. (C) 2015 Elsevier Ltd. All rights reserved.
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