Journal
BIORESOURCE TECHNOLOGY
Volume 194, Issue -, Pages 67-74Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.06.087
Keywords
Bacillus sp.; Pineapple; Poly(3-hydroxybutyrate); Statistical test; Sugar-based material
Funding
- Thailand Research Fund (TRF) for The Royal Golden Jubilee (RGJ) Fund, Bangkok, Thailand [PHD/0163/2553]
- Thailand Research Fund (TRF), Bangkok, Thailand [DBG 5380013]
- Graduate School of Khon Kaen University, Khon Kaen University
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According to the cost of carbon substrate for producing biopolymer of polyhydroxyalkanoates (PHAs) still has a barrier to extend in an industrial scale. The aim of this work was to evaluate the defined PHAs media containing the agricultural raw materials of pineapple and sugarcane to produce PHAs by Bacillus strain. Batch fermentation was carried out in flask scale to compare the efficiency of defined media using statistical methodology. The defined medium 8 of pineapple was achieved the highest PHAs concentration and productivity (1.86 g/L and 0.077 g/Lh). The effect of pH and aeration was extensively studied in a fermentor. The results were revealed that PHAs production would be increased from the condition of uncontrolled pH and at higher aeration rate. The extracted PHAs clearly showed in a homopolymer structure of poly(3-hydroxybutyrate) (PHB) with melting temperature (T-m) of 172 degrees C 54.39% crystallinity. (C) 2015 Published by Elsevier Ltd.
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