Journal
BRITISH JOURNAL OF BIOMEDICAL SCIENCE
Volume 73, Issue 2, Pages 74-78Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09674845.2016.1162376
Keywords
Calcitriol; vitamin D; inflammation; septic shock; defensins; endothelial activation
Categories
Funding
- Mexican Institute for Social Security-IMSS [FIS/IMSS/PRIO13/025]
Ask authors/readers for more resources
Background: Calcitriol (vitamin D) supplementation has been proposed for therapeutical use in vascular diseases due to its immunomodulatory activity, preventing inflammation and promoting angiogenesis. In the present study, we hypothesised whether calcitriol downregulates pro-inflammatory gene expression without affecting angiogenesis and anti-inflammatory gene expression in LPS-induced endothelial cells.Method: In order to evaluate the effect of calcitriol in suppressing inflammatory gene expression in the endothelium, endothelial cells were exposed to the physiological concentration of calcitriol followed by stimulation with lipopolysaccharide (LPS). Gene expression of interleukin (IL)-1, Transforming Growth Factor (TGF)-, Human -defensin (HBD)-2, angiogenin (ANG) and cathelicidin (LL-37) were quantified by quantitative polymerase chain reaction.Results: The results from six independent experiments conducted in duplicate, showed that calcitriol decreased IL-1 (p<0.01) and HBD-2 expression (p<0.01) when compared to non-treated cells. However, calcitriol treatment had no effect on TGF-, ANG and LL-37 gene expression.Conclusion: Calcitriol prevents inflammatory gene expression, but does not affect expression of angiogenic genes in endothelial cells, which suggest the potential use of calcitriol to prevent endothelial activation through the downregulation of IL-1 and HBD-2.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available