Journal
HYPERTENSION
Volume 75, Issue 1, Pages 79-90Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/HYPERTENSIONAHA.119.13173
Keywords
chromatin immunoprecipitation; heart; hypertrophy; mice; rats
Categories
Funding
- National Key RD Program [2017YFC0909301]
- National Natural Science Foundation of China [81570354, 81470511, 81770394, 81770392, 81370338, 81700344, 81800344, 81500361, 81800345]
- Science and Technology Project of Guangdong Province and Guangzhou [201610010125]
- Science and Technology Program Foundation of Guangdong [2017A020215156]
- Guangdong Natural Science Foundation [2016A030310180, 2017A030313813]
- Fundamental Research Funds for the Central Universities [17ykpy27]
- Science Foundation of Guangdong Second Provincial General Hospital [YQ2018-012]
- Doctoral Workstation Foundation of Guangdong Second Provincial General Hospital [2019BSGZ004]
- Medical Research Foundation of Guangdong Province [A2018107, A2018082]
Ask authors/readers for more resources
Transcribed ultraconserved regions (T-UCRs) are a novel class of long noncoding RNAs transcribed from UCRs, which exhibit 100% DNA sequence conservation among humans, mice, and rats. However, whether T-UCRs regulate cardiac hypertrophy remains unclear. We aimed to explore the effects of T-UCRs on cardiac hypertrophy. First, we performed long noncoding RNA microarray analysis on hearts of mice subjected to sham surgery or aortic banding and found that the T-UCR uc.323 was decreased significantly in mice with aortic banding-induced cardiac hypertrophy. In vitro loss- and gain-of-function experiments demonstrated that uc.323 protected cardiomyocytes against hypertrophy induced by phenylephrine. Additionally, we discovered that mammalian target of rapamycin 1 contributed to phenylephrine-induced uc.323 downregulation and uc.323-mediated cardiomyocyte hypertrophy. We further mapped the possible target genes of uc.323 through global microarray mRNA expression analysis after uc.323 knockdown and found that uc.323 regulated the expression of cardiac hypertrophy-related genes such as CPT1b (Carnitine Palmitoyl transferase 1b). Then, chromatin immunoprecipitation proved that EZH2 (enhancer of zeste homolog 2) bound to the promoter of CPT1b via H3K27me3 (trimethylation of lysine 27 of histone H3) to induce CPT1b downregulation. And overexpression of CPT1b could block uc.323-mediated cardiomyocyte hypertrophy. Finally, we found that uc.323 deficiency induced cardiac hypertrophy. Our results reveal that uc.323 is a conserved T-UCR that inhibits cardiac hypertrophy, potentially by regulating the transcription of CPT1b via interaction with EZH2.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available