Journal
HUMAN IMMUNOLOGY
Volume 81, Issue 2-3, Pages 101-104Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2020.01.009
Keywords
CD4(+) T cell; Activation; Siglec-10; Protein; RNA; CD52
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Funding
- Australian National Health and Medical Research Council (NHMRC) [10373210]
- NHMRC Senior Principal Research Fellowship [1080887]
- National Health and Medical Research Council of Australia [1080887] Funding Source: NHMRC
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Most sialic acid-binding immunoglobulin-like lectins (Siglecs) suppress immune cell function but are expressed at low levels on human T cells. We found that soluble CD52 inhibited T cell signalling by ligating Siglec-10, but the presence of Siglec-10 on human T cells has been questioned. To address this concern, we examined the expression of Siglec-10 at the RNA and protein level in human CD4(+) T cells. Analysis by RNAseq, qPCR and flow cytometry demonstrated that, in contrast to other Siglecs, after activation of CD4(+) T cells Siglec-10 was selectively upregulated in a subset of cells also high for CD52 expression. This observation is consistent with a homeostatic role for Siglec-10 in human CD4(+) T cells.
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