Journal
BIORESOURCE TECHNOLOGY
Volume 190, Issue -, Pages 57-65Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.03.114
Keywords
Integrated biorefinery; Crude glycerol; Sunflower meal; Oleaginous yeast; Bioprocess
Funding
- BIOREF'' project [09SYN-81-715]
- National (Greek Ministry-General Secretariat of Research and Technology)
- Community Funds (E.U.-European Social Fund)
- Spanish Ministry of Economy and Competitiveness [ENE2010-15159, ENE2013-47769-R]
- Andalusian Research, Innovation and Enterprise Council, Spain [TEP-4994]
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This study focuses on the valorisation of crude glycerol and sunflower meal (SFM) from conventional biodiesel production plants for the separation of value-added co-products (antioxidant-rich extracts and protein isolate) and for enhancing biodiesel production through microbial oil synthesis. Microbial oil production was evaluated using three oleaginous yeast strains (Rhodosporidium toruloides, Lipomyces starkeyi and Cryptococcus curvatus) cultivated on crude glycerol and nutrient-rich hydrolysates derived from either whole SFM or SFM fractions that remained after separation of value-added co-products. Fed-batch bioreactor cultures with R. toruloides led to the production of 37.4 g L-1 of total dry weight with a microbial oil content of 51.3% (w w(-1)) when a biorefinery concept based on SFM fractionation was employed. The estimated biodiesel properties conformed with the limits set by the EN 14214 and ASTM D 6751 standards. The estimated cold filter plugging point (7.3-8.6 degrees C) of the lipids produced by R. toruloides is closer to that of biodiesel derived from palm oil. (C) 2015 Elsevier Ltd. All rights reserved.
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