Journal
BIORESOURCE TECHNOLOGY
Volume 196, Issue -, Pages 383-390Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.07.088
Keywords
Endophytes; Enzymatic saccharification; Ascomycetes; Dothideomycetes; Pretreatment
Funding
- Spanish Ministry of Economy and Competitiveness [CTQ 2011-28503-C02-01, CTQ2013-47158-R, PTA 2011-4857-I]
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New endophytic fungi are assessed for the first time as pretreatment to enhance saccharification of Eucalyptus globulus wood. The fungi are all laccase-producing ascomycetes and were isolated from eucalyptus trees in Spain. After five endophytes had been assayed alone or in combination with white-rot fungus Trametes sp. I-62, three were pre-selected. To improve sugar production, an autohydrolysis pretreatment was performed before or after fungal treatment. Pretreatment increased sugar production 2.7 times compared to non-pretreated wood. When fungal and autohydrolysis pretreatments were combined, a synergistic increase in saccharification was observed in all cases. Endophytic fungi Ulocladium sp. and Hormonema sp. produced greater enhancements in saccharification than Trametes sp. I-62 (increase in sugar yields of 8.5, 8.0 and 6.0 times, respectively), demonstrating the high potential of these new endophytic fungi for saccharification enhancement. (C) 2015 Elsevier Ltd. All rights reserved.
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