Journal
FOOD CHEMISTRY
Volume 302, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125349
Keywords
Critical interfacial concentration; Protein-stabilized emulsion; FTIR subtraction method; Second derivative spectra; Pendant drop analysis; Extrinsic fluorescence
Funding
- German Research Foundation Priority Program 1934, DispBiotech
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Knowledge about the critical interfacial concentration of a protein supports our understanding of the kinetic stability of an emulsion. Its determination is currently limited to either invasive or indirect methods. The aim of our study was the determination of the critical interfacial concentration of whey protein beta-lactoglobulin at oil/water-interfaces through fluorescence and pendant drop analysis and the comparison to an in situ Fouriertransform-infrared-spectroscopy (FTIR) method. Exponentially decreasing interfacial tension with increasing beta-lactoglobulin content (0.10-1.00 wt%) in pendant drop analysis could partly be confirmed by fluorescence spectra. A critical interfacial concentration of 0.20-0.31 wt% beta-lactoglobulin (1.80-2.69 mg/m(2)) in oil/water (5/95)-emulsions was determined via FTIR, analyzing the Amide I/Amide II peak intensity ratio. This was confirmed by the increasing formation of intermolecular beta-sheets, revealed by second derivative spectra. With this FTIR method we expand current options to investigate the interfacial behavior of food proteins by determination of secondary structure elements.
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