Journal
FOOD CHEMISTRY
Volume 309, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125710
Keywords
Glucose isomerase; Enzyme immobilization; Sepabeads; Batch reactor; Packed bed reactor; HFS
Funding
- Tunisian Ministry of Higher Education and Scientific Research and Technology [LR15CBS06]
- University of Sfax (doctoral school of FSS)
- Spanish Ministry of Economy and competitiveness [BIO2016-76601-C3-1-R]
- CONACYT (Mexico)
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The glucose isomerase GICA from Caldicoprobacter algeriensis was immobilized by ionic adsorption on polymethacrylate carriers (Sepabeads EC-EA and EC-HA) or covalent attachment to glyoxal agarose. The Sepabeads EC-HA yielded the highest recovery of activity (89%). The optimum temperature and pH of immobilized GICA were 90 degrees C and 7.0, respectively, similar to the corresponding values of free enzyme. Nevertheless, the adsorbed enzyme displayed higher relative activity at acidic pH, greater thermostability, and better storage stability, compared to the free form. Moreover, the immobilized enzyme showed an excellent operational stability, in 15 successive 3 h reaction cycles at 85 degrees C under a batch reactor, preserving 83% of its initial activity. Interestingly, a continuous process for High Fructose Syrup (HFS) production was established with the adsorbed GICA using a packed bed reactor during eleven days at 70 degrees C. HPAEC-PAD analysis showed a maximum bioconversion rate of 49% after 48 h of operation.
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