Journal
BIORESOURCE TECHNOLOGY
Volume 187, Issue -, Pages 173-181Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.03.117
Keywords
Protein purification; Glycosyl hydrolases; Mixture experimental design; Enzymatic hydrolysis; Sugar cane bagasse
Funding
- Sao Paulo Research Foundation (FAPESP) [2008/57873-8, 2010/08089-2, 2012/23223-2]
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The aim of this work was to study cocktail supplementation for sugar cane bagasse hydrolysis, where the enzymes were provided from both commercial source and microorganism cultivation (Trichoderma reesei and genetically modified Escherichia coli), followed by purification. Experimental simplex lattice mixture design was performed to optimize the enzymatic proportion. The response was evaluated through hydrolysis microassays validated here. The optimized enzyme mixture, comprised of T. reesei fraction (80%), endoglucanase (10%) and beta-glucosidase (10%), converted, theoretically, 72% of cellulose present in hydrothermally pretreated bagasse, whereas commercial Celluclast 1.5 L converts 49.11% +/- 0.49. Thus, a rational enzyme mixture designed by using synergism concept and statistical analysis was capable of improving biomass saccharification. (C) 2015 Elsevier Ltd. All rights reserved.
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