Journal
FOOD CHEMISTRY
Volume 303, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125375
Keywords
Graphene nanoplatelets; Graphitized nanodiamonds; Urea; Sensing; Screen-printed electrode
Funding
- Nanotechnology: Impact on Safety, Health and Environment Program (CSIR-NANOSHE)
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT, & Future Planning, South Korea [2016R1E1A1A01940995]
- Department of Science and Technology, New Delhi, India
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Urea is well-known to offer tremendous scope for sensing/diagnosing such as adulteration in dairy products or diseases in human body. This study was organized to describe and validate a new mediator-free, unsophisticated, and direct current voltage (IV)-based sensor for facile detection of urea using nanocomposites made of urease-immobilized graphene nanoplatelets and graphitized nanodiamonds. This nanocomposite displayed sensitive and direct signal in the form of current at 0 V without the need of any complex chemical reaction. This platform was highly sensitive (limit of detection of 5 mu g/mL) far superior to the comparable systems introduced recently. The incorporation of graphitized nanodiamonds within the graphene nanoplatelets layers helped improve the sensitivity by a factor of three (up to 806.3 mu A (mg mL(-1))(-1) cm(-2)) with 20 s response time. As such, the use of this nanocomposite was helpful in improving sensing performances with enhanced enzyme loading capacity.
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