Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 136, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2019.111058
Keywords
Gatifloxacin; Bovine serum albumin; Metal complex; Toxicity; Spectroscopic approaches
Categories
Funding
- National Key Research and Development Program [2017YFC1601202]
- National Natural Science Foundation of China [21804029, 21976129, 21777165]
- Natural Science Foundation of Anhui Province, China [1908085QB67]
- China Postdoctoral Science Foundation [2019M651770]
- Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University [KLSAOFM1811]
- Shandong Key Laboratory of Biochemical Analysis [SKLBA1904]
- Open Project of Faculty of Chemistry of Qingdao University of Science and Technology [QUSTHX201805]
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Fluoroquinolone antibiotics (FQs), a new class of pollutants that seriously threaten human health through environmental and food residues, have aroused wide public concern. However, little attention has been paid to the potential toxicity of FQs' metal complex. Here, we firstly explore the proof-of-concept study of FQs' metal complex to bind bovine serum albumin (BSA) using systematical spectroscopic approaches. In detail, we have found that the complex of Al3+ with gatifloxacin (Al(III)-GFLX complex) can effectively bind to BSA via electrostatic interaction in PBS buffer (pH = 7.4, 1 x), resulting in the formation of Al(III)-GFLX-BSA complex. The negative value of Delta G shows that the binding of Al(III)-GFLX complex to BSA is a spontaneous process. Circular dichroism spectra verify that Al(III)-GFLX complex effectively triggers the conformation changes of BSA's secondary structure. It has been proved that the interaction of small molecule with serum albumin has a significant effect on their in vivo biological effects such as absorption, distribution, metabolism, and excretion, and etc. Therefore, the results of this paper may offer a valuable theoretical basis for establishing safety standards of FQs' metal complex to ensure food and environmental health.
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