Journal
FOOD AND AGRICULTURAL IMMUNOLOGY
Volume 31, Issue 1, Pages 379-392Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2020.1733495
Keywords
Lactococcus lactis; DCpep; immune response; efficacy
Funding
- National Natural Science Foundation of China [31101318, 31973003]
- Natural Science Foundation of Heilongjiang Province [C201422]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars by State Education Ministry [20151098]
- State Education Ministry
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In the present study, recombinant L. lactis NZ9000 displaying anchored dendritic cell-targeting peptide (DCpep) and E. tenella 3-1E protein was constructed. Chickens were orally immunized with the recombinant lactococci for three times at 2-week intervals. After three immunizations, chickens were challenged with E. tenella sporulated oocyst, and the immune response and protective efficacy were evaluated. The finding from animal experiments showed recombinant lactococci expressing anchored DCpep-3-1E protein elicited higher levels of 3-1E specific serum IgG and secretory IgA (sIgA) in caecal lavage, higher proportions of CD4+ and CD8 alpha+ cells in peripheral blood, and higher mRNA expression levels of IL-2 and IFN-gamma in the spleen compared to 3-1E-expressing lactococci, and hence offered obvious protective efficacy against homologous challenge. The present work demonstrated that cell surface-displayed target protein and immune enhancers in lactococci may be a promising approach to enhance immunity and immune efficacy against pathogen infection.
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