Journal
BIORESOURCE TECHNOLOGY
Volume 194, Issue -, Pages 326-333Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.07.026
Keywords
Microalgae; Wastewater; Semi-continuous culture; Biomass; Lipid
Funding
- Ministry of Science and Technology [MOST 103-3113-E-006-006]
- Aim for the Top University Program of the National Chiao Tung University
- Ministry of Education, Taiwan
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The development of a culture system for Chlorella sp. GD to efficiently produce biomass and oil for biodiesel production was investigated. Chlorella sp. GD was cultivated with 0%, 25%, 50%, 75% and 100% piggery wastewater (diluted by medium) at 300 mu mol m(-2) s(-1), a 2% CO2 aeration rate of 0.2 vvm and 26 +/- 1 degrees C; after a 10-day culture in batch cultures, the maximum specific growth rate and biomass productivity of the microalga obtained in 100% piggery wastewater were 0.839 d(-1) and 0.681 g L-1 d(-1), respectively. The highest lipid content and lipid productivity were 29.3% and 0.155 g L-1 d(-1) at 25% wastewater, respectively. In semi-continuous cultures, the biomass and lipid productivities with 25-75% wastewater ratios were greater than 0.852 and 0.128 g L-1 d(-1), respectively. These results show that Chlorella sp. GD grows efficiently in piggery wastewater, and that a stable growth performance was achieved for long-term microalgal cultivation in a semi-continuous culture. (C) 2015 Elsevier Ltd. All rights reserved.
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