Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 95, Issue -, Pages 506-513Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.10.073
Keywords
Heat shock proteins; Embryonic development; Gene expression; Cellular response; Dojo loath
Funding
- National Natural Science Foundation of China [31572592]
- Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province [2014154]
- Science and Technology Key Project of Changsha [ZD1601003]
Ask authors/readers for more resources
As molecular chaperones, heat shock proteins (HSPs) play essential roles in cells in response to stress conditions. Recent studies about immune functions of HSPs in fish have also been reported. In this study, based on the reported cDNA sequences of the four HSP genes, HSP70, HSC70, HSP90 alpha and HSP90 beta, the temporal expression patterns of the four genes during embryonic development of dojo loach(Misgurnus anguillicaudatus) was assayed with qRT-PCR. All of the four genes were ubiquitously expressed in all detected embryonic developmental stages. Among of them, HSP70, HSC70 and HSP90 beta were highly expressed in the organ formation stage, while HSP90a was the highest expressed in myotome formation stage. Further, the immune responses of the four HSP genes were assayed when loath were infected with three different pathogens, bacterium (Flavobacterium cloumnare G4), parasite (Ichthyophthirius multifiliis) and fungus (Saprolegnia). All of the four genes were differentially expressed in four tissues such as skin, gills, spleen and kidney in response to the pathogenic invasion, but both HSP70 and HSP90 alpha expressions were dramatically up-regulated. Further, the cellular responses of the loath skin and gill tissues were observed, in which the number of the skin goblet cells were significantly increased, and the gill lamellae became shorter and wider after infected. Thus, our work indicated that the HSPs may directly or indirectly involved in immune defense in fish, at least in the loath.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available