Journal
EUROPEAN JOURNAL OF PHYCOLOGY
Volume 55, Issue 3, Pages 275-284Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09670262.2019.1709131
Keywords
Brown algae; cell wall; embryogenesis; Fucus; haloperoxidase; hydrogen peroxide; phloroglucinol; phlorotannins; zygote
Categories
Funding
- Russian Foundation for Basic Research [17-04-01331]
- University of Leipzig
- University of St. Petersburg
Ask authors/readers for more resources
Vanadium-dependent haloperoxidases (V-HPO) are specific enzymes involved in the metabolism of reactive oxygen species in marine algae. These enzymes contribute to early embryogenesis of algae in the order Fucales, catalyzing the reactions which result in phlorotannin incorporation into the newly forming zygote cell wall and adhesive material. In this study we showed that early embryogenesis in Fucus vesiculosus is accompanied by synchronous changes of V-HPO activity, hydrogen peroxide content and content of cell-wall-associated phlorotannins. Phlorotannin incorporation into the cell wall of Fucus zygotes had already begun 1 h after fertilization, and this process was accompanied by rapid increase of H2O2 content and V-HPO activity. We suggest that altogether this initiates polymerization of phenolics and their cross-linking with the major cell wall constituent, alginic acid. Presumably, de novo phlorotannin biosynthesis is involved in this process, as GC-MS analysis showed dramatic changes in content of intracellular phloroglucinol and low-molecular-weight phlorotannins during the first 9 days of F. vesiculosus embryogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available