Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 50, Issue 3, Pages 353-362Publisher
WILEY
DOI: 10.1002/eji.201948342
Keywords
conditional mutagenesis; CreER; fate mapping; microglia; recombination
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Funding
- Israel Science Foundation [887/11]
- European Research Council [Adv ERC 340345]
- International Progressive MS Alliance (PMSA)
- Deutsche Forschungsgemeinschaft (DFG) [CRC/TRR167]
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Conditional mutagenesis and fate mapping have contributed considerably to our understanding of physiology and pathology. Specifically, Cre recombinase-based approaches allow the definition of cell type-specific contributions to disease development and of inter-cellular communication circuits in respective animal models. Here we compared Cx(3)cr1(CreER) and Sall1(CreER) transgenic mice and their use to decipher the brain macrophage compartment as a showcase to discuss recent technological advances. Specifically, we highlight the need to define the accuracy of Cre recombinase expression, as well as strengths and pitfalls of these particular systems that should be taken into consideration when applying these models.
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