Journal
BIORESOURCE TECHNOLOGY
Volume 197, Issue -, Pages 369-374Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.07.094
Keywords
Extractability; Microalgae; Fatty acids; Flow cytometry; Screening
Funding
- project PSE-microalgas (Spanish Ministry of Science and Innovation)
- project ALGAFEED (Canarias Objetivo de Progreso programme, ERDF)
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The lipid extractability of 14 microalgae species and strains was assessed using organic solvents (methanol and chloroform). The high variability detected indicated the potential for applying this parameter as an additional criterion for microalgae screening in industrial processes such as biofuel production from microalgae. Species without cell walls presented higher extractability than species with cell walls. Analysis of cell integrity by flow cytometry and staining with propidium iodide showed a significant correlation between higher resistance to the physical treatments of cell rupture by sonication and the lipid extractability of the microalgae. The results highlight the cell wall as a determining factor in the inter-and intraspecific variability in lipid extraction treatments. (C) 2015 Published by Elsevier Ltd.
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