Journal
ENVIRONMENT INTERNATIONAL
Volume 136, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envint.2020.105497
Keywords
Natural sphalerite; Antibiotic-resistance genes; Antibiotic-resistant bacteria; Conjugative transfer; Stress response; Cell membrane permeability
Categories
Funding
- NSFC [41573086, 41425015, U1901210]
- Science and Technology Project of Guangdong Province, China [2017A050506049]
- Leading Scientific, Technical and Innovation Talents of Guangdong Special Support Program [2016TX03Z094]
- Research Grant Council of Hong Kong SAR Government [GRF14100115]
Ask authors/readers for more resources
Minerals and microorganisms are integral parts of natural environments, and they inevitably interact. Antibiotic-resistance genes (ARGs) significantly threaten modern healthcare. However, the effects of natural minerals on ARG propagation in aquatic systems are not fully understood. The present work studied the effects of natural sphalerite (NS) nanoparticles on the horizontal transfer of ARGs from Escherichia coil DH5 alpha (CTX) (donor) to E. coli. C600 (Sm) (recipient), and from E. coli. DH5 alpha (MCR) (donor) to E. coli. C600 (Sm), and their underlying mechanisms. NS particles (0.5-50 mg L-1) induced an NS-concentration-dependent increase in conjugative transfer frequency. The underlying mechanisms associated with the facilitated ARG transfer included the production of intracellular reactive oxygen species, the SOS response, changes in bacterial cell morphology, and alteration of mRNA levels of bacterial cell membrane protein-related genes and genes associated with conjugative ARG transfer. The information herein offers new mechanistic understanding of risks of bacterial resistance resulting from NS.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available