Journal
ELECTROPHORESIS
Volume 41, Issue 7-8, Pages 545-553Publisher
WILEY
DOI: 10.1002/elps.201900321
Keywords
ESI; Free-flow zone electrophoresis; Matrix effects; Microfluidic; Serum sample
Funding
- Defense Threat Reduction Agency (DTRA) [HDTRA1-09-C-0013]
- National Science Foundation [CHE-1808507]
- Wyoming INBRE program (National Institutes of Health) [P20GM103432]
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Matrix components are known to significantly alter the ionization of a target analyte in ESI-based measurements particularly when working with complex biological samples. This issue however may be alleviated by extracting the analyte of interest from the original sample into a relatively simple matrix compatible with ESI mass-spectrometric analysis. In this article, we report a microfluidic device that enables such extraction of small peptide molecules into an ESI-compatible solvent stream significantly improving both the sensitivity and reproducibility of the measurements. The reported device realizes this analyte extraction capability based on the free-flow zone electrophoretic fractionation process using a set of internal electrodes placed across the width of the analysis channel. Employing lateral electric fields and separation distances of 75 V/cm and 600 mu m, respectively, efficient extraction of the model peptide human angiotensin II was demonstrated allowing a reduction in its detection limit by one to three orders of magnitude using the ESI-MS method. The noted result was obtained in our experiments both for a relatively simple specimen comprising DNA strands and angiotensin II as well as for human serum samples spiked with the same model peptide.
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