Journal
DYES AND PIGMENTS
Volume 171, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2019.107755
Keywords
H2S-Specific; Two nucleophilic; Ca2+ mediated; Vivo
Funding
- National Natural Science Foundation of China [21775096, 21302223]
- Shanxi Province 1331 project key innovation team construction plan cultivation team [2018-CT-1]
- Shanxi Province Foundation for Returness [2017-026]
- Shanxi Province Science Foundation for Youths [201701D221061]
- Scientific Instrument Center of Shanxi University [201512]
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Hydrogen sulfide (H2S), a new endothelium-derived relaxing factor (EDRF), which plays vital roles in regulating intracellular redoxstatus and other fundamental signaling processes involved in human health and disease. In this work, we designed a fluorescent probe for H2S successive nucleophilic reaction with high signal-to-background (S/B) ratio. In the probe, we utilized 2-mercaptobenzoic acid as the distinguish reaction site and introduced 2,4-dinitrophenyl to provide appropriate steric hindrance to realize specific response on well-structured H2S. Other substances sulfur-containing do not disturb the detection of H2S. The max emission with 197-fold enhancement exhibits high signal-to-background ratio. The detection limit was calculated to be 64 nM. Imaging assays in living cells showed that the probe could penetrate cells membrane easily and allowed to detect endogenous generation H2S induced by Ca2+ mediated cystathionine gamma-lyase (CSE). Moreover, the probe was successfully used to detect exogenous H2S in mice.
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