Journal
BIORESOURCE TECHNOLOGY
Volume 192, Issue -, Pages 367-373Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.05.093
Keywords
Sugarcane bagasse; Glycerol; Pretreatment; Enzymatic hydrolysis; Fermentation
Funding
- Australian Government Sugar Research and Development Corporation
- Syngenta Biotechnology Inc., USA
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In this study, for the first time the effects of glycerol on enzymatic hydrolysis and ethanol fermentation were investigated. Enzymatic hydrolysis was inhibited slightly with 2.0 wt% glycerol, leading to reduction in glucan digestibility from 84.9% without glycerol to 82.9% (72 h). With 5.0 wt% and 10.0 wt% glycerol, glucan digestibility was reduced by 4.5% and 11.0%, respectively. However, glycerol did not irreversibly inhibit cellulase enzymes. Ethanol fermentation was not affected by glycerol up to 5.0 wt%, but was inhibited slightly at 10.0 wt% glycerol, resulting in reduction in ethanol yield from 86.0% in the absence of glycerol to 83.7% (20 h). Based on the results of laboratory and pilot-scale experiments, it was estimated that 0.142 kg ethanol can be produced from 1.0 kg dry bagasse (a glucan content of 38.0%) after pretreatment with acidified glycerol solution. (C) 2015 Elsevier Ltd. All rights reserved.
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