Journal
DEVELOPMENTAL CELL
Volume 52, Issue 4, Pages 461-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2019.12.010
Keywords
-
Categories
Funding
- Natural Sciences and Engineering Council of Canada [RGPIN-2017-05861]
- Canadian Institutes of Health Research [MOP79497]
- Michael Smith Foundation for Health Research
- Canada Foundation for Innovation
- British Columbia Knowledge Development Fund
- Natural Sciences and Engineering Council of Canada NSERC Alexander Graham Bell Canada Graduate Scholarship
- European Molecular Biology Organization long-term fellowship
- MRC [MC_UU_00012/6] Funding Source: UKRI
Ask authors/readers for more resources
Phosphoinositides, diacylglycerolpyrophosphate, ceramide-1-phosphate, and phosphatidic acid belong to a unique class of membrane signaling lipids that contain phosphomonoesters in their headgroups having pK(a) values in the physiological range. The phosphomonoester headgroup of phosphatidic acid enables this lipid to act as a pH biosensor as changes in its protonation state with intracellular pH regulate binding to effector proteins. Here, we demonstrate that binding of pleckstrin homology (PH) domains to phosphatidylinositol 4-phosphate (PI4P) in the yeast trans-Golgi network (TGN) is dependent on intracellular pH, indicating PI4P is a pH biosensor. pH biosensing by TGNPI4P in response to nutrient availability governs protein sorting at the TGN, likely by regulating sterol transfer to the TGN by Osh1, a member of the conserved oxysterol-binding protein (OSBP) family of lipid transfer proteins. Thus, pH biosensing by TGN PI4P allows for direct metabolic regulation of protein trafficking and cell growth.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available