4.5 Review

Organic phase separation opens up new opportunities to interrogate the RNA-binding proteome

Journal

CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 54, Issue -, Pages 70-75

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2020.01.009

Keywords

RNA-binding proteins; Organic phase separation; Transcriptome; Mass spectrometry; RNA-sequencing

Funding

  1. Wellcome Trust, United Kingdom [110070/Z/15/Z, 110071/Z/15/Z]
  2. University of Cambridge, United Kingdom, Herchel Smith Research Studentship
  3. German Research Foundation (DFG) [IRTG 2290]
  4. Plus 3 Programme of the Boehringer Ingelheim Foundation (BIS), Germany
  5. Joachim Herz Foundation, Germany
  6. Excellence Cluster Cell Networks
  7. Wellcome Trust [110071/Z/15/Z] Funding Source: Wellcome Trust
  8. BBSRC [BB/N010493/1] Funding Source: UKRI
  9. MRC [MC_UP_A600_1023, MC_UU_00025/7] Funding Source: UKRI
  10. Biotechnology and Biological Sciences Research Council [BB/M006700/1] Funding Source: researchfish
  11. Medical Research Council [MC_UP_A600_1023, MC_UU_00025/7] Funding Source: researchfish
  12. Wellcome Trust [110071/A/15/Z] Funding Source: researchfish

Ask authors/readers for more resources

Protein-RNA interactions regulate all aspects of RNA metabolism and are crucial to the function of catalytic ribonucleoproteins. Until recently, the available technologies to capture RNA-bound proteins have been biased toward poly(A) RNA-binding proteins (RBPs) or involve molecular labeling, limiting their application. With the advent of organic-aqueous phase separation-based methods, we now have technologies that efficiently enrich the complete suite of RBPs and enable quantification of RBP dynamics. These flexible approaches to study RBPs and their bound RNA open up new research avenues for systems-level interrogation of protein-RNA interactions.

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