Journal
BIORESOURCE TECHNOLOGY
Volume 192, Issue -, Pages 689-695Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.06.037
Keywords
Neutral red; Electron transfer; Bioelectrochemical system; Microbial electrosynthesis; Menaquinone
Funding
- NSF [0954186]
- NIH [5T32GM008336-24]
- Department of Energy's Office of Biological and Environmental Research
- Linus Pauling Distinguished Postdoctoral Fellowship at Pacific Northwest National Laboratory
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0954186] Funding Source: National Science Foundation
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The aim of this work was to elucidate the mechanism of mediated microbial electrosynthesis via neutral red from an electrode to fermenting Escherichia coli cultures in a bioelectrochemical system. Chemical reduction of NAD+ by reduced neutral red did not occur as predicted. Instead, neutral red was shown to reduce the menaquinone pool in the inner bacterial membrane. The reduced menaquinone pool altered fermentative metabolite production via the arcB redox-sensing cascade in the absence of terminal electron acceptors. When the acceptors DMSO, fumarate, or nitrate were provided, as many as 19% of the electrons trapped in the reduced acceptors were derived from the electrode. These results demonstrate the mechanism of neutral red-mediated microbial electrosynthesis during fermentation as well as how neutral red enables microbial electrosynthesis of reduced terminal electron acceptors. (C) 2015 Elsevier Ltd. All rights reserved.
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