Journal
BIORESOURCE TECHNOLOGY
Volume 192, Issue -, Pages 228-237Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.05.065
Keywords
Elephant grass; Steam explosion; Enzymatic hydrolysis; Fermentation; Cellulosic ethanol
Funding
- UCS
- CAPES
- CNPq [310590/2009-4]
- FAPERGS [10/1972-5]
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In this work, steam explosion was used a pretreatment method to improve the conversion of elephant grass (Pennisetum purpureum) to cellulosic ethanol. This way, enzymatic hydrolysis of vaccum-drained and water-washed steam-treated substrates was carried out with Penicillium echinulatum enzymes while Saccharomyces cerevisiae CAT-1 was used for fermentation. After 48 h of hydrolysis, the highest yield of reducing sugars was obtained from vaccum-drained steam-treated substrates that were produced after 10 min at 200 degrees C (863.42 +/- 62.52 mg/g). However, the highest glucose yield was derived from water-washed steam-treated substrates that were produced after 10 min at 190 degrees C (248.34 +/- 6.27 mg/g) and 200 degrees C (246.00 +/- 9.60 mg/g). Nevertheless, the highest ethanol production was obtained from water-washed steam-treated substrates that were produced after 6 min at 200 degrees C. These data revealed that water washing is a critical step for ethanol production from steam-treated elephant grass and that pretreatment generates a great deal of water soluble inhibitory compounds for hydrolysis and fermentation, which were partly characterized as part of this study. (C) 2015 Elsevier Ltd. All rights reserved.
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