Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 186, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2019.110683
Keywords
Biosensor; MoS2 nanosheets; Gold nanoparticles; Laccase; Catechol detection
Funding
- national first-class discipline program of Light Industry Technology and Engineering (LITE2018-21) [LITE2018-21]
- Natural Science Foundation of Jiangsu Province [BK20180628]
- National Science Foundation of China [51803078]
- 111 Project [B17021]
- Fundamental Research Funds for the Central Universities [JUSRP11701]
- Top-notch Academic Programs Project of Jiangsu Higher Education Institutions [PPZY2015B147]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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As a kind of two-dimensional layered nanomaterial, MoS2 nanosheets have been widely used in the field of biosensors. In this work, a novel laccase based biosensor for catechol detection was fabricated based on a nanocomposite of MoS2 nanosheets and gold nanoparticles. The experimental results demonstrated that MoS2 had large specific surface area and good biocompatibility, which provided abundant position for enzyme immobilization. Gold nanoparticles enhanced conductivity of MoS2 and improved detection sensitivity. Because of the synergistic effect of MoS2 nanosheets and gold nanoparticles, the lactase based bioelectrode exhibited a linear response to catechol from 2 to 2000 mu M with a detection limit of 2 mu M (S/N = 3), as well as good selectivity, stability, repeatability, and reproducibility.
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