Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 186, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2019.110721
Keywords
Sensitivity improvement; Signal amplification; Photothermal quantification; Lateral flow assay; Reduced graphene oxide
Funding
- Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
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Lateral flow immunoassay (LFA) is a well-known point-of-care technology for the detection of various analytes. However, low sensitivity and lack of quantitative results are some of its critical drawbacks. Here we report a photothermal enhanced lateral flow sensor on the basis of the photothermal properties of reduced graphene oxide (rGO) for the detection of E-coli O157:H7 as a model pathogen. The calibration curve of the photothermal method exhibited a linear range from 5 x 10(5) to 5 x 10(7) CFU/ml with a correlation coefficient of R-2 = 0.96 and a regression equation of y = 8.1x-43 for standard bacteria solutions in phosphate buffer. The limit of detection was similar to 5 x 10(5) CFU/ml for standard bacteria solutions, which was a 10-fold enhancement in sensitivity compared to the qualitative results. Specificity experiments showed that the photothermal method can only detect the target bacteria among 6 types of bacteria strains. It was confirmed that the developed technique could be a highly potential method for the rapid detection field because it can provide fast quantitative results with improved sensitivity.
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