Journal
CLINICAL INFECTIOUS DISEASES
Volume 72, Issue 5, Pages 806-813Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/cid/ciaa159
Keywords
Clostridioides difficile; whole-genome sequencing; Clostridium difficile; ribotype; clinical outcomes
Categories
Funding
- National Institutes of Health [DK114007]
- Center for Individualized Medicine at the Mayo Clinic
Ask authors/readers for more resources
Through WGS analysis of C. difficile isolates from CDI patients, it was found that 40% of community-acquired CDI patients may have transmission within the same zip code. WGS better differentiates between relapse and reinfection than definitions based on the timing of recurrence.
Background. Current approaches in tracking Clostridioides difficile infection (CDI) and individualizing patient management are incompletely defined. Methods. We recruited 468 subjects with CDI at Mayo Clinic Rochester between May and December 2016 and performed whole-genome sequencing (WGS) on C. difficile isolates from 397. WGS was also performed on isolates from a subset of the subjects at the time of a recurrence of infection. The sequence data were analyzed by determining core genome multilocus sequence type (cgMLST), with isolates grouped by allelic differences and the predicted ribotype. Results. There were no correlations between C. difficile isolates based either on cgMLST or ribotype groupings and CDI outcome. An epidemiologic assessment of hospitalized subjects harboring C. difficile isolates with allelic differences, based on standard infection prevention and control assessment, revealed no evidence of person-to-person transmission. Interestingly, community-acquired CDI subjects in 40% of groups with <= 2 allelic differences resided within the same zip code. Among 18 subjects clinically classified as having recurrent CDI, WGS revealed 14 with initial and subsequent isolates differing by allelic differences, suggesting a relapse of infection with the same initial strain, and 4 with isolates differing by >50 allelic differences, suggesting reinfection. Among the 5 subjects classified as having a reinfection based on the timing of recurrence, 3 had isolates with <= 2 allelic differences between them, suggesting a relapse, and 2 had isolates differing by >50 allelic differences, suggesting reinfection. Conclusions. Our findings point to potential transmission of C. difficile in the community. WGS better differentiates relapse from reinfection than do definitions based on the timing of recurrence.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available