Journal
CHROMATOGRAPHIA
Volume 83, Issue 3, Pages 467-475Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-019-03853-3
Keywords
Liquid chromatography-tandem mass spectrometry; Glyphosate; Aminomethylphosphonic acid; Method validation; Heptafluorobutyric acid
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A method for the simultaneous determination of glyphosate and aminomethylphosphonic acid (AMPA) in urine was developed using a dilute-and-shoot sample preparation. A 1:1 dilution of urine sample in 15 mmol L-1 heptafluorobutyric acid (HFBA) was prepared prior to being injected into the LC-MS/MS system. The chromatographic separation was achieved by a Gemini C6-Phenyl analytical column, with the gradient elution of 15 mmol L-1 HFBA in water and acetonitrile. The limits of detection and quantitation of both compounds were 2.5 ng mL(-1) and 5 ng mL(-1), respectively. The sample preparation time was achieved in 2 min using only 0.1 mL of urine sample. The validated method was successfully applied to glyphosate and AMPA determination in farmer urine samples. Graphic
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