Journal
CHEMOSPHERE
Volume 240, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2019.124951
Keywords
Microextraction by packed sorbent; Endocrine-disrupting chemicals; Liquid chromatography-tandem mass spectrometry; Human urine samples; Biomonitoring studies
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Funding
- Sao Paulo Research foundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP) [2018/24069-3, 2016/02949-6, 2014/19914-5, 2014/50867-3, 2013/23710-3]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior Brasil (CAPES) [001]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
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A high demand exists in human biomonitoring studies for reliable and straightforward methods that generate data faster and simultaneously. Thus, the present study combines microextraction by packed sorbent (MEPS) and liquid chromatography coupled to mass spectrometry (LC-MS/MS) for simultaneous extraction and determination of various classes of endocrine-disrupting chemicals (EDCs), including parabens, benzophenones, bisphenols, and the antimicrobial, triclocarban in human urine samples. Optimized MEPS conditions were: i) MEPS sorbent (C18), ii) pH of sample (3), iii) volume of sample (250 mu L), iv) number of draws-eject cycles (5) and (vi) desorption solvent conditions (100 mu L of CH3OH:H2O 80:20 v/v). The calibration curves were linear over the selected ranges for all studied compound, with correlation coefficients higher than 0.99. The variation coefficient for precision was lower than 20% at lower concentrations and lower than 15% at the higher concentrations studied. The accuracy ranged from 90% to 118%. The proposed strategy affords several advantages over currently published approaches, including simplicity of operation and reduction of sample and solvent volumes and time for matrix clean-up. Moreover, the analytical performance of each MEPS cartridge remained stable over the analysis of at least 70 samples (RSD < 10%). Thus, the current procedure may be an interesting high-throughput alternative for large routine human biomonitoring studies. Urinary geometric mean concentrations of EDCs obtained in this study were close than those previously reported for Brazilian children. (C) 2019 Elsevier Ltd. All rights reserved.
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