Journal
CHEMISTRY LETTERS
Volume 49, Issue 2, Pages 145-148Publisher
CHEMICAL SOC JAPAN
DOI: 10.1246/cl.190804
Keywords
Nuclear proteomics; Photoactivatable proximity labeling; Protein chemical modification
Categories
Funding
- Japan Science and Technology Agency (JST) ERATO Grant [JPMJER1802]
- [18K14334]
- [19H05764]
- [17H06348]
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The precise characterization of organellar proteomes is essential for deciphering a variety of cellular functions at the molecular level. We here report a photoactivatable proximity labeling method for identifying organellar proteomes with high spatiotemporal resolution. In our strategy, a photosensitizer targeted to the nucleus of live cells is irradiated with light to locally generate singlet oxygen. An o-phenylenediamine-based substrate then promiscuously tags nearby proteins. This method coupled with LC-MS/MS allowed the specific identification of nuclear proteins, including typical nuclear proteins such as histones and lamins.
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