Distance-dependent Fluorescence Quenching on a Silver Nanoparticle Surface

Distance dependent fluorescence quenching and mechanisms are studied on a silver nanoparticle (AgNP) surface. The longer the distance between fluorophore and AgNP surface, the less quenching. The relationship fits the quantum efficiency energy transfer equation where a Nanoparticle-Based Energy Transfer (NSET) quenching model dominates with the distance dependent factor of d(4.42) and an estimated NSET radius of 14.4 nm. Within a 10 nm range, the quenching is a result of the combination of Forster Resonance Energy Transfer and NSET.