Journal
CHEMBIOCHEM
Volume 21, Issue 9, Pages 1285-1287Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900716
Keywords
AMPylation; click chemistry; fluorescent probes; post-translational modifications; proteomics
Funding
- European Research Council (ERC) [725085-CHEMMINE, SFB749]
- Alexander von Humboldt fellowship
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Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.
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